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MATSUSHIMA Ayami

 
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NameMATSUSHIMA Ayami
URLhttp://lsfb.scc.kyushu-u.ac.jp/
AffiliationKyushu University
SectionGraduate School of Sciences, Department of Molecular Chemistry, Laboratory of Structure-Function Biochemistry
Job titleassociate professor
DegreeScience(Kyushu University)
Other AffiliationKyushu University Risk Science Research Center

Research Interests

 
environmental chemicals , opioid receptor , estrogen receptor , Peptide

Research Areas

 
  • Boundary medicine / Pain science / 
  • Applied chemistry / Bio-related chemistry / 
  • Biomolecular science / Biomolecular chemistry / 
  • Biological Science / Functional biochemistry / 
  • Biological Science / Structural biochemistry / 
  • Environmental science / Risk sciences of radiation and chemicals / 

Education

 
 
 - 
2001
Graduate School, Division of Natural Science, Kyushu University
 
 
 - 
2004
分子科学専攻, Graduate School, Division of Natural Science, Kyushu University
 
 
 - 
1999
Faculty of Science, Kyushu University
 

Published Papers

 
Ayami Matsushima
International Journal of Molecular Sciences   19    May 2018
© 2018 by the author. Licensee MDPI, Basel, Switzerland. Huge numbers of chemicals are released uncontrolled into the environment and some of these chemicals induce unwanted biological effects, both on wildlife and humans. One class of these chemi...
LIU Xiaohui, 松島綾美, 下東康幸
月刊細胞   49(7) 363‐367   Jun 2017
Xiaohui Liu, Hirokazu Nishimura, Akina Fujiyama, Ayami Matsushima, Miki Shimohigashi, Yasuyuki Shimohigashi
Biopolymers   547-554   Nov 2016
© 2015 Wiley Periodicals, Inc. Estrogen-related receptor γ (ERRγ) is a constitutively active nuclear receptor functioning as a transcription factor. ERRγ binds to a single half site designated as ERRE that has only a single DNA-binding motif. Howe...
Ayami Matsushima, Hirokazu Nishimura, Yutaka Matsuyama, Xiaohui Liu, Tommaso Costa, Yasuyuki Shimohigashi
Biopolymers   460-469   Nov 2016
© 2016 Wiley Periodicals, Inc. We previously showed that an antagonist-based peptide ligand, H-Cys(Npys)-Arg-Tyr-Tyr-Arg- Ile-Lys-NH2, captures the free thiol groups in the ligand-binding site of the nociceptin receptor ORL1. However, the exact re...
Shogo Inamine, Hirokazu Nishimura, Jinglan Li, Kaname Isozaki, Ayami Matsushima, Tommaso Costa, Yasuyuki Shimohigashi
Bioorganic and Medicinal Chemistry   22 5902-5909   Nov 2014
© 2014 Elsevier Ltd. All rights reserved. IsoVa-RYYRIK-NH2is a highly specific antagonist ligand of the opioid receptor-like 1 (ORL1) receptor, an endogenous ligand of which is 17-mer peptide nociceptin. ORL1 antagonists have potential for clinica...
Jinglan Li, Hirokazu Nishimura, Ayami Matsushima, Yasuyuki Shimohigashi
Bioorganic and Medicinal Chemistry   22 5721-5726   Nov 2014
© 2014 Elsevier Ltd. All rights reserved. Antagonists of the neuropeptide nociceptin are expected to be potential analgesic and antineuropathic drugs acting on ORL1 GPCR receptors. The peptide library-based antagonist Ac-RYYRIK-NH2inhibits the noc...
Xiaohui Liu, Ayami Matsushima, Miki Shimohigashi, Yasuyuki Shimohigashi
PLoS ONE   9    Jun 2014
The endocrine disruptor bisphenol A (BPA) affects various genes and hormones even at merely physiological levels. We recently demonstrated that BPA binds strongly to human nuclear receptor estrogen-related receptor (ERR) γ and that the phenol-A gr...
Ayami Matsushima, Kerrianne Ryan, Yasuyuki Shimohigashi, Ian A. Meinertzhagen
Environ. Pollut.   173 257-263   Feb 2013   [Refereed]
Bisphenol A (BPA) is widely used industrially to produce polycarbonate plastics and epoxy resins. Numerous studies document the harmful effects caused by low-dose BPA exposure especially on nervous systems and behavior in experimental animals such...
Liu X, Matsushima A, Nakamura M, Costa T, Nose T, Shimohigashi Y
J. Biochem.   151 403-415   Apr 2012   [Refereed]
Various lines of evidence have shown that bisphenol A (BPA) acts as an endocrine disruptor that affects various hormones even at merely physiological levels. We demonstrated recently that BPA binds strongly to human nuclear receptor estrogen-relat...
Matsushima, A., Nishimura, H., Inamine, S., Uemura, S., Shimohigashi, Y.
Bioorg. Med. Chem.   19(24) 7597-7602   Dec 2011   [Refereed]
All of the δ, μ, and κ opioid receptors have a free thiol group of the Cys residue in the ligand-binding site, although its functional role is not yet known. In order to examine whether or not a similar Cys is also present in the ORL1 nociceptin r...
Arase, S., Ishii, K., Igarashi, K., Aisaki, K., Yoshio, Y., Matsushima, A., Shimohigashi ,Y., Arima, K., Kanno, J., Sugimura, Y.
Bio. Reprod.   84 734-742   Apr 2011   [Refereed]
The balance between androgens and estrogens is very important in the development of the prostate, and even small changes in estrogen levels, including those of estrogen-mimicking chemicals, can lead to serious changes. Bisphenol A (BPA), an endocr...
Hassaneen, E., El-Din Sallam, A., Abo-Ghalia, A., Moriyama, Y., Karpova, S.G., Abdelsalam, S., Matsushima, A., Shimohigashi, Y., and Tomioka, K.
J. Biol. Rhythm   26 3-13   Feb 2011   [Refereed]
Pigment-dispersing factor (PDF) is a neuropeptide widely distributed in insect brains and plays important roles in the circadian system. In this study, we used RNA interference to study the role of the pigment-dispersing factor (pdf) gene in regul...
Liu, X., Matsushima, A., Okada, H., and Shimohigashi, Y.
J. Biochem   148(2) 247-254   2011   [Refereed]
Bisphenol A (BPA) strongly binds to human estrogen-related receptor γ (ERRγ). BPA is an oestrogenic endocrine disruptor that influences various physiological functions at very low doses. BPA functions as an inverse-type antagonist of ERRγ to retai...
Matsushima, A., Liu, X., Okada, H., Shimohigashi, M., and Shimohigashi, Y
Environ. Health Perspect.   118(9) 1267-1272   Sep 2010   [Refereed]
Background: Bisphenol AF has been acknowledged to be useful for the production of CF3-containing polymers with improved chemical, thermal, and mechanical properties. Because of the lack of adequate toxicity data, bisphenol AF has been nominated fo...
Kaname Isozaki, Jinglan Li, Kazushi Okada, Hirokazu Nishimura, Ayami Matsushima, Takeru Nose, Tommaso Costa, Yasuyuki Shimohigashi
Bioorganic and Medicinal Chemistry   17 7904-7908   Dec 2009
[Arg14,Lys15]Nociceptin is a very potent for ORL1 receptor, showing a few times stronger binding activity and much more enhanced biological activity than endogenous nociceptin. This synergistic outcome has been suggested to be due to the interacti...
Hirokazu Nishimura, Jinglan Li, Kaname Isozaki, Kazushi Okada, Ayami Matsushima, Takeru Nose, Tommaso Costa, Yasuyuki Shimohigashi
Bioorganic and Medicinal Chemistry   17, 5683-5687   Aug 2009
ORL1 is an endogenous G protein-coupled receptor for neuropeptide nociceptin. [(R/K)14, (R/K)15]nociceptin is a superagonist that strongly activates the ORL1 receptor. We have previously found that substituting with Trp can reproduce the potentiat...
Yukimasa Takeda, Yukimasa Takeda, Xiaohui Liu, Miho Sumiyoshi, Ayami Matsushima, Miki Shimohigashi, Yasuyuki Shimohigashi
Journal of Biochemistry   146(1) 113-122   Jul 2009
Estrogen-related receptor γ (ERRγ), one of the 48 human nuclear receptors, has a fully active conformation with no ligand. We recently demonstrated that ERRγ binds strongly bisphenol A (BPA), one of the nastiest endocrine disruptors, and thus reta...
Kazushi Okada, Kaname Isozaki, Jinglan Li, Ayami Matsushima, Takeru Nose, Tommaso Costa, Yasuyuki Shimohigashi
Bioorganic and Medicinal Chemistry   16 9261-9267   Oct 2008
Nociceptin is an endogenous ligand that activates a G protein-coupled receptor ORL1 and contains two indispensable Arg-Lys (RK) dipeptide units at positions 8-9 and 12-13. By replacing an additional RK unit at positions 6-7, 10-11, 14-15, or 16-17...
Satoru Yokotani, Takeru Nose, Yuji Horiuchi, Ayami Matsushima, Yasuyuki Shimohigashi, Yasuyuki Shimohigashi
Protein and Peptide Letters   15 949-955   Sep 2008
Analysis of the amino acid composition of prion protein using a newly developed program for radar-chart deviation analysis has identified an abnormality or irregularity of the N-terminal flexible domain. Aromatic amino acids Trp and His together w...
Ayami Matsushima, Takamasa Teramoto, Hiroyuki Okada, Xiaohui Liu, Takatoshi Tokunaga, Yoshimitsu Kakuta, Yasuyuki Shimohigashi
Biochemical and Biophysical Research Communications   373(3) 408-413   Aug 2008
A receptor-binding assay and X-ray crystal structure analysis demonstrated that the endocrine disruptor bisphenol A (BPA) strongly binds to human estrogen-related receptor γ (ERRγ). BPA is well anchored to the ligand-binding pocket, forming hydrog...
Jinglan Li, Kaname Isozaki, Kazushi Okada, Ayami Matsushima, Takeru Nose, Tommaso Costa, Yasuyuki Shimohigashi
Bioorganic and Medicinal Chemistry   16 2635-2644   Mar 2008
Nociceptin is an endogenous agonist ligand of the ORL1 (opioid receptor-like 1) receptor, and its antagonist is a potential target of therapeutics for analgesic and antineuropathy drugs. Ac-RYYRIK-NH2is a hexapeptide isolated from the peptide libr...
Hiroyuki Okada, Takatoshi Tokunaga, Xiaohui Liu, Sayaka Takayanagi, Ayami Matsushima, Yasuyuki Shimohigashi
Environmental Health Perspectives   116 32-38   Jan 2008
Background: Various lines of evidence have shown that bisphenol A [BPA; HO-C6H4-C(CH3)2- C6H4-OH] acts as an endocrine disruptor when present in very low doses. We have recently demonstrated that BPA binds strongly to human estrogen-related recept...
Design Synthesis of Highly Potent Antagonist of ORL1 Nociceptin Receptor.
Li, J. Isozaki, K., Okada, K., Matsushima, A., Nose, T., Costa, T., and Shimohigashi, Y.
Bioorg. Med. Chem.      2008
Xiaohui Liu, Ayami Matsushima, Hiroyuki Okada, Takatoshi Tokunaga, Kaname Isozaki, Yasuyuki Shimohigashi, Yasuyuki Shimohigashi
FEBS Journal   274 6340-6351   Dec 2007
Bisphenol A, 2,2-bis(4-hydroxyphenyl)propane, is an estrogenic endocrine disruptor that influences various physiological functions at very low doses, even though bisphenol A itself is ineffectual as a ligand for the estrogen receptor. We recently ...
Ayami Matsushima, Yoshimitsu Kakuta, Takamasa Teramoto, Takumi Koshiba, Xiaohui Liu, Hiroyuki Okada, Takatoshi Tokunaga, Shun Ichiro Kawabata, Makoto Kimura, Yasuyuki Shimohigashi
Journal of Biochemistry   142(4) 517-524   Oct 2007
Many lines of evidence reveal that bisphenol A (BPA) functions at very low doses as an endocrine disruptor. The human estrogen-related receptor γ (ERRγ) behaves as a constitutive activator of transcription, although the endogenous ligand is unknow...
Ayami Matsushima, Katsuhiro Takano, Taichi Yoshida, Yukimasa Takeda, Satoru Yokotani, Yasuyuki Shimohigashi, Miki Shimohigashi
Journal of Biochemistry   141(6) 867-877   Jun 2007
Many lines of evidence have suggested that neuropeptides other than pigment-dispersing factor (PDF) are involved in regulating insect circadian rhythms, and FMRFamide-related peptides are additional candidates acting as such neuromodulators. Doubl...
Sayaka Takayanagi, Takatoshi Tokunaga, Xiaohui Liu, Hiroyuki Okada, Ayami Matsushima, Yasuyuki Shimohigashi
Toxicology Letters   167 95-105   Dec 2006
Bisphenol A (BPA) has been acknowledged as an estrogenic chemical able to interact with human estrogen receptors (ER). Many lines of evidence reveal that BPA has an impact as an endocrine disruptor even at low doses. However, its binding to ER and...
Takeshi Honda, Takeshi Honda, Ayami Matsushima, Kazunori Sumida, Yoshiro Chuman, Yoshiro Chuman, Kazuyasu Sakaguchi, Kazuyasu Sakaguchi, Hitoshi Onoue, Ian A. Meinertzhagen, Yasuyuki Shimohigashi, Yasuyuki Shimohigashi, Miki Shimohigashi, Miki Shimohigashi
Journal of Comparative Neurology   499 404-421   Nov 2006
Pigment-dispersing factor (PDF) is an 18-mer peptide that acts as a principal neurotransmitter of the insect circadian clock. Our previous study, utilizing anti-Uca β-PDH polyclonal antibody (pAb) to immunolabel the optic lobe of the cricket Gryll...
Molecular Cloning and Circadian Expression Profile of Pacemaker Neuropeptide PDF in Diptera
Matsushima, S., Yokotani, S., Sato, S., Kaneki, A., Takeda, Y., Chuman, Y., Ozaki, M., Asai, D., Nose, T., Onoue, H., Ito, Y., Tominaga, Y., Shimohigashi, Y., and Shimohigashi, M.
Lett., Peptide Sci.      2005
Yukimasa Takeda, Yoshiro Chuman, Naoto Shirasu, Seiji Sato, Ayami Matsushima, Atsushi Kaneki, Yoshiya Tominaga, Yasuyuki Shimohigashi, Miki Shimohigashi
Zoological Science   21 903-915   2005
The molecular basis of the circadian clock is an autoregulatory feedback loop in which the PAS domain-containing protein PERIOD periodically inhibits its own transcription. In the present study on PERIOD of the silk moth Bombyx mori, we have clone...
Ayami Matsushima, Seiji Sato, Yoshiro Chuman, Yukimasa Takeda, Satoru Yokotani, Takeru Nose, Yoshiya Tominaga, Miki Shimohigashi, Yasuyuki Shimohigashi
Journal of Peptide Science   10 82-91   Feb 2004
Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. Recently, two completely different types of PDF precursor were clarified; that ...
Ayami Matsushima, Satoru Yokotani, Xiaohui Lui, Kazunori Sumida, Takeshi Honda, Seiji Sato, Atsushi Kaneki, Yukimasa Takeda, Yoshiro Chuman, Yoshiro Chuman, Mamiko Ozaki, Daisuke Asai, Takeru Nose, Hitoshi Onoue, Yushi Ito, Yoshiya Tominaga, Yasuyuki Shimohigashi, Miki Shimohigashi
Letters in Peptide Science   10 419-430   Nov 2003
Pigment-dispersing factor PDF is an 18-amino acid insect neuropeptide that mediates a circadian rhythmicity in locomotor activity. PDF is coded in a precursor protein together with another neuropeptide named PDF-associated peptide, PAP. PDF is hig...
Seiji Sato, Yoshiro Chuman, Ayami Matsushima, Yoshiya Tominaga, Yasuyuki Shimohigashi, Miki Shimohigashi
Zoological Science   19(8) 821-828   Aug 2002
Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neuromodulator functioning downstream of the insect brain's circadian clock, modulating daily rhythms of locomotor activity. Recently, we found that PDF precu...
Yoshiro Chuman, Ayami Matsushima, Seiji Sato, Kenji Tomioka, Yoshiya Tominaga, Ian A. Meinertzhagen, Yasuyuki Shimohigashi, Miki Shimohigashi
Journal of Biochemistry   131 895-903   Jan 2002
Pigment-dispersing factor (PDF) was recently reported to be a principal circadian neuromodulator involved in transmitting circadian rhythms of daily locomotion in insects. In Drosophila, PDF functions in some of the neurons expressing the clock ge...
A. Matsushima, T. Fujita, K. Okada, N. Shirasu, T. Nose, Y. Shimohigashi
Bulletin of the Chemical Society of Japan   73(11) 2531-2538   Nov 2000
The thrombin receptor-tethered ligand SFLLRNP (abbreviation formed by one letter amino acid codes expressing Ser-Phe-Leu-Leu-Arg-Asn-Pro) consists of the Phe-2 residue essential for the receptor activation. In order to explore the molecular charac...
Tsugumi Fujita, Takeru Nose, Ayami Matsushima, Kazushi Okada, Daisuke Asai, Yasuko Yamauchi, Naoto Shirasu, Takeshi Honda, Daiki Shigehiro, Yasuyuki Shimohigashi
Tetrahedron Letters   41(6) 923-927   Feb 2000
A complete set of difluorophenylalanines in the L-configuration [L- (F2)Phe] (namely, L-(2,3-F2)Phe, L-(2,4-F2)Phe, L-(2,5-F2)Phe, L-(2,6- F2)Phe, L-(3,4-F2)Phe, L-(3,5-F2)Phe) was prepared and incorporated into the thrombin receptor-tethered liga...
A. Matsushima, T. Fujita, T. Nose, Y. Shimohigashi
Journal of Biochemistry   128 225-232   Jan 2000
In the ligand/receptor interaction, the side chain phenyl group of phenylalanine (Phe) is involved in a so-called hydrophobic interaction, in which the Phe-phenyl group functions as a π element or merely as a hydrophobic element. The thrombin rece...
Edge-to-face CH/π Interaction between Ligand Phe-phenyl and Receptor Aromatic Group in Thrombin Receptor Activation (共著)
J.Biochem   225-232    2000

Misc

 
Phe 2715.39 of Thronbin Receptor PAR-1 is a specific target of Phe-2-phenl group of its tethered ligand
Sugiyama, M., Fujita, T., Liu, X., Yutaka, M., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2017      2018   [Refereed]
Mutual co-work between nuclear receptor ER and ERR requires their homodimerization
Liu, X., Shimohigashi, M., Sugiyama, M., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2017      2018   [Refereed]
Homodimer function of human nuclear receptor ERR evidenced using -helix peptides in the dimer interface
Liu, X., Nakagawa, H., Sugiyama, M., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2017      2018   [Refereed]
SCN Circadian Rhythm Signal Transduction Neuropeptides Evoke Hypoactive Phenotype Differently in Male and Female Mice.
Sugiyama, M., Motomatsu, Y., Matsuyama, Y., Kajiyama, S., Kameda, T., Saito, T., Uchimura, E., Liu, X., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2015      2016   [Refereed]
ORL1 Nociceptin Receptor Response of Opioid Peptides Derived from Proenkephalin Precursor Protein.
Motomatsu, Y., Nishimura, H., Matsusyama, Y., Liu, X., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2015      2016   [Refereed]
Functional Role of the Phe-Phe Stacking Microswitch in the ORL1 Nociceptin Receptor Activation.
Takesue, Y., Nishimura, H., Liu, X., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2015      2016   [Refereed]
Expression Analysis of Neuropeptide PDF mRNA in the Hypoactive and Hyperactive Fruit Flies Drosophila melanogaster.
Tokumaru, A., Matsuo, A., Umeno, S., Matsuyama, Y., Nakamura, M., Sumiyoshi, M., Liu, X., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2015      2016   [Refereed]
Do the Estrogen Receptors Function as a Homodimer? Experimental Validation of Homodimerization by Inhibitory Peptides.
Sakito, S., Liu, X., Fujiyama, A., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2015      2016   [Refereed]
Comprehensive structural analysis of the nociceptin ORL1 receptor by means of virtual and experimental Ala-scanning combinated methods.
Matsushima, A., Nishiimura, H., Liu, X., Takesue, Y., Matsuyama, Y., and Shimohigashi. Y.
Peptide Science 2015,      2016   [Refereed]
Comparative Analysis of the Ligand Binding Domain Secondary Structures of Human Nuclear Receptors.
Matsuyama, Y., Nishimura H., Liu, X., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2015      2016   [Refereed]
The molecular switching of ORL1 nociceptin receptor in activation/inactivation.
Nishimura, H., Li, J., Isozaki, K., Takesue, Y., Liu, X., Shimohigashi, M., Inamine, S., Matsushima, A., and Shimohigashi. Y.:
Peptide Science 2014      2015   [Refereed]
Structural and molecular evolutionary analysis of the ligand-binding domain of forty-eight human nuclear receptors
Matsuyama, Y., Nishimura H., Liu, X., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2014      2015   [Refereed]
GPCR functional role of Phe-269 and Phe-221 in the molecular switching of ORL1 nociceptin receptor activation.
Takesue, Y., Nishimura, H., Liu, X., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2014      2015   [Refereed]
Bisphenol A-induced substantial peak decay of Drosophila circadian neuropeptide hugin mRNA expression.
Umeno, S., Matsuo, A., Matsuyama, Y., Nakamura, M., Koga, K., Liu, X., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2014      2015   [Refereed]
An in silico genomic search of endomorphin-like opioid peptides
Matsushima, A., Koyanagi, O.K., Nishimura, H., Inamine, S., Motomatsu, Y., and Shimohigashi. Y.
Peptide Science 2014      2015   [Refereed]
Alternative polyadenylation analyses of neuropeptide genes in bisphenol A-exposed hypoactive mouse brain.
Sugiyama, M., Kajiyama, S., Saito, T., Uchimura, E., Motomatsu, Y., Matsuo, A., Matsushima, A., and Shimohigashi. Y.
Peptide Science 2014      2015   [Refereed]
A novel method to identify and Quantify the coactivator proteins that couple with human nuclear receptor: the use of interacting interface a-helix peptide for quantative inhibition
Liu, X., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2014      2015   [Refereed]
Structure-activity Studies on the Halogenated Phe-containing Neuropeptide Substance P Analogs.
Kuramitsu, Y., Nishimura, H., Nakamura, R., Suyama, K., Matsushima, A., Nose, T., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Specific Role of Phe Residues in Opioid Peptide Met-enkephalin- Arg-Phe in Binding to All Three Opioid Receptors.
Motomatsu, Y., Nishimura, H., Matsumoto, Y., Inamine, S., Kuramitsu, Y., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Multiple and Simultaneous Cys→Ala Mutations of ORL1 Nociceptin Receptor to Identify the Affinity Binding site of Cys(Npys)-Elongated RYYRIK Peptide Antagonist.
Matsushima, A., Nishimura, H., Inamine, S., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Influence Analysis of the Circadian Evening Pacemaker Neuropeptide hugγ Gene in the Bisphenol A-exposed Fruit Fly Drosophila Brain.
Umeno, S., Matsuo, A. , Matsuyama, Y., Nakamura, M., Takeda, Y., Sumiyoshi, M., Liu, X., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Functionally Essential Amino Acid Residues in ORL1 Nociceptin Receptor for Ligand-stimulated Receptor Activation.
Nishimura, H., Li, J., Isozaki, K., Abe, Y., Inamine, S., Matsushima, A., Costa, T., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Expression and Mutation Analyses of Brain Neuropeptide Genes of Rndocrine-disrupting Bisphenol A-exposed Hypoactive Mouse.
Sugiyama, M., Matsuo, A., Saito, T., Uchimura, E., Liu, X., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Constitutive α-Helix-peptides Required for Functional Dimerization of Estrogen-related Receptor γ (ERRγ).
Liu, X., Nishimura, H., Fujiyama, A., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Bisphenol A-induced Epigenetic Mutations in Circadian Pacemaker Neuropeptide mRNAs of Hyperactive Drosophila Fruit Flies.
Matsuo, A., Umeno, S., Matsuyama, Y., Nakamura, M., Takeda, Y., Sumiyoshi, M., Liu, X., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Three-dimensional Docking Modeling to Human Nuclear Receptors for Exploration of Bisphenol-A Targeting Receptors.
Matsuyama, Y., Liu, X., Nishimura, H., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2013      2014   [Refereed]
Two different binding modes of peptide library-based antagonist acyl-RYYRIK amide in the orl1 nociceptin receptor.
Inamine, S., Nishimura, H., Li, J., Matsushima, A. Costa, T., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
The impact of bisphenol A-feeding on the Drosophila neuropeptide PDF mRNA structure.
Matsuo, A., Nakamura, M., Takeda, Y., Matsuyama, Y., Sumiyoshi, M., Liu, X., Matsushima, A. Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
Structural characteristics of Thr-Gly dipeptide repeat in the Drosophila clock protein PERIOD.
Matsuo, A., Nishimura, H., Nakamura, M., Koga, K., Sumiyoshi, M., Matsushima, A., Nose, T., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
Identification of affinity binding site of Cys(Npys)-elongated RYYRIK peptide antagonist by means of Cys→Ala mutated ORL1 nociceptin receptors.
Matsushima, A., Nishimura, H., Inamine, S., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
High-precision binding assay procedure of tachykinin receptor NK-1 for highly potent Substance P analogs.
Kuramitsu, Y., Nishimura, H., Nakamura, R., Suyama, K., Matsushima, A., Nose, T., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
Halogenated Phe-containing endomorphin-2 analogs with mixed agonist and antagonist activities.
Nishio, K., Nishimura, H., Suyama, K., Matsushima, A., Nose, T., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
Exploration of pharmacological chaperone for rescue of misfolded and/or unfolded ORL1 nociceptin receptor proteins.
Nishimura, H., Inamine, S., Li, J. Matsushima, A., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
Bisphenol-binding pocket of constitutively active nuclear receptor CAR: Docking modeling for close-packing.
Matsuyama, Y., Liu, X., Nishimura, H., Matsushima, A., Nose, T., and Shimohigashi, Y.
Peptide Science 2012      2013   [Refereed]
QCM-analyses of the intermolecular interaction between prion protein N-terminal Domains having increased number of octapeptide repeat
Sato, K., Horiuchi, Y., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2011      2011   [Refereed]
The Trp residue of opioid receptor TM5 present at the cell membrane interface is a molecular anchor for full activation.
Nishimura, H., Li, J., Inokuchi, N., Koikawa, S., Matsushima, A., Nose, T., Costa, T. and Shimohigashi, Y.
Peptide Science 2010      2011   [Refereed]
Exploration of the binding site of ORL1 nociceptin receptor antagonist.
Inamine, S., Li, J., Nishimura, H., Matsushima, A., Nose, T., Costa, T. and Shimohigashi, Y.
Peptide Science 2010      2011   [Refereed]
The functional role of Trp present at the cell membrane interface in the opioid receptor activation.
Nishimura, H., Li, J., Inokuchi, N., Koikawa, A., Matsuhima, A., Nose, T., Costa, T., and Shimohigashi, Y.
Peptide Science 2009      2010
The direct measure of intermolecular interaction of prion protein N-terminal octapeptide repeats by the quarts crystal microbalance (QCM).
Horiuchi, Y., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2009      2010
Shimohigashi, M., Itoh, T.Q., Honda, T., Saito, M., Koga, K., Takahashi, K., Matsushima, A., Ueda, R., Tanimura T., Shimohigashi, Y., and Matsumoto A.
Horiuchi, Y., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2009      2010
Structure-function analysis of nociceptin receptor ORL1 by the site-directed mutagenesis
Li, J., Isozaki, K., Matsushima, A., Nose, T., Costa, T., and Shimoohigashi, Y.
Peptide Science 2009      2010
The preparation and purification of prion protein N-terminal domain with fluctuations in the number of octapeptide repeat
Sato, K., Horiuchi, Y., Matsushima, A., and shimohigashi, Y.
Peptide Science 2009      2010
Functional role of the C-terminal Helix 12 peptide in the receptor activation mechanism of estrogen-related receptor γ (ERRγ).
Liu, X., Matsushima, A., Okada, H., and Shimohigashi, Y.
Peptide Science 2009      2010
Induced-fit type ligand binding guided by free-rotatory Leu residue present in the 7th α-helix peptide in the estrogen-related receptor γ (ERRγ)
Matsushima, A., Okada, H., Liu, X., Tokunaga, T., Teramoto, T., Kakuta, Y., Shimohigashi, Y.
Peptide Science 2008      2009
Molecular recognition of prion protein via its N-terminal octapeptide repeat Structure
Horiuchi, Y., Hattori, E., Yokotani, S., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2008      2009
ERα/ERRα Nuclear Receptor Heterodimer Directly Linked by A Flag Peptide.
Ikeda, S., Matsushim, A., and Shimohigashi, Y.
Peptide Science 2008      2009
Bisphenol A-specific nuclear receptor ERRγ: structure-function analysis of the two novel isoforms lacking vital peptide fragment in the ligand binding domain
Takeda, Y., Liu, X., Sumiyoshi, M., Matsushima, A., Shimohigashi, Y. and Shimohigashi, M.
Peptide Science 2008      2009
Optimization of the N-Terminal Group of Ac-RYYRIK-NH2 as ORL1 Receptor Antagonist
Li, J. Isozaki, K., Matsushima, A., Nose, T., Costa, T., and Shimohigashi, Y.
Peptide Science 2007      2008
The Output Mechanism of Circadian Pacemaker Neuropeptide PDF in the Regulation of Bimodal Locomotor Distribution
Takeda, Y., Koga, K., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2007      2008
Structural Requirement of Housefly FMRFamide Peptides in Its Receptor Activation.
Matsushima, A., Koretsune, Y., Kaneki, A., Isozaki, K., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2007      2008
The Effect of Amino Acid Substitution on Oligomerization of Octapeptide Repeat Structure in Prion Protein.
Hattori, E., Yokotani, S., Horiuchi, Y., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2007      2008
A strategy to explore the target receptor of endocrine disruptors: Estrogen-related receptor γ (ERRγ) as a genuine acceptor of bisphenol A.
Matsushima, A., Shimohigashi, Y.
Alternatives to Animal Testing and Experimentation.AATEX journal, 14, special issue,      2008
Immunological evidences of circadian neuropeptide PDF isoform present at the lamina outer cells and varicose network in the optic lobes of the cricket Gryllus bimaculatus
Honda, T., Matsushima, A., Hiramura, D., Sumida, K., Takeda, Y., Sato, S., Meinertzhagen I.A., Shimohigashi, Y., and Shimohigashi, M.,
Peptide Science 2005      2006
Specific dimerization of prion protein N-terminal domain
Matsushima, A., Horiuchi, Y., Yokotani, S., Kawano, M., and Shimohigashi, Y.
Peptide Science 2005      2006
Identification of novel isoforms of the circadian neuropeptide PDF in the silk moth Bombyx mori and their expression in brain
Sato, S., Jonathan C., Corrins, Takeda, Y., Kaneki, A., Matsushima, A., Shimohigashi, Y., and Shimohigashi, M.
Peptide Science 2005      2006
a-helix peptides for bio-panning in the phage display method to obtain the antibodies specific for conformation-change nuclear receptors
Okada, H., Tokunaga, T., Shirasu N., Matsushima, A., Nose, T., and Shimohigashi Y.,
Peptide Science 2005      2006
Design and synthesis of a-helix peptide binding to prion protein N-terminal octarepeat domain
Koga, K., Sato S., Horiuchi Y., Matsushima, A., and Shimohigashi, Y.
Peptide Science 2005      2006
Conformation Change of α-Helix Peptide for Sensing of Deactivation of Nuclear Receptor: Immunoassay Using Polyclonal Antibody Specific for the C-terminal α-Helix 12 of Estrogen-related Receptor γ (ERRγ)
Tokunaga, T., Liu, X., Okada, H., Matsushima, A., Nose, T., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2006      2006
Molecular Cloning and in situ Hybridization of Circadian Neuropeptide PDF Receptor in the Fruit Fly Drosophila melanogaster
Kaneki, A., Yoshida, T., Isozaki, K., Matsushima A., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2006      2006
Immunological Confirmation of Circadian-related Neuropeptide PDF-Isoform Peptide in the Cricket Gryllus bimaculatus
Koga, K., Yakayama, Y., Hiramura, D., Takeda, Y., kaneki, A., Matsushima, A., Shimohigashi, Y., and Shimoshigashi, M.
Peptide Science 2006      2006
Multiple Post-transcriptional Regulations in Circadian Pacemaker Neuropeptide pdf Gene
Takeda, Y., Sumiyuki, M., Koga, K., Ito, M., Matsushima, A., Shimohigashi, Y., and Shimohigashi, M.
Peptide Science 2006      2006
Structure-Activity Studies of FMRFamide-Related Peptides in Activating the Specific Receptor Present in the Housefly Musca domestica
Matsushima, A. Koretsune, Y., Kaneki, A. Isozaki, K., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2006      2006
FMRFamide-Related Peptides in the Nervous System of the Housefly Musca domestica: cDNA Cloning and Actions on Clam Heart Contraction
Matsushima ., Yokotani, S., Koretsune, Y., Meinertzhagen, I.A., Tominaga, Y., Shimohigashi, M., and Shimohigashi, Y.
Peptide Science 2004      2005
Structural Characteristics of the N-Terminal Octapeptide Repeat Region of Prion Protein in Self-Polymerization
Horiuchi, Y., Kawano, M., Yokotani, S., Honda, T., Matsushima, A., Nose, T., and Shimohigashi, Y.
Peptide Science 2004      2005
Bioactive Conformation of a D-Trp-Containing Cardioexcitatory Tripeptide Isolated from the Sea Hare Aplysia
okotani, S., Matsushima, A., Nose, T., Morishita, F., and Shimohigashi Y.
Peptide Science 2004      2005
cDNA Cloning and mRNA Expression of the Circadian Neuropeptide PDF in the Silk Moth Bombyx mori
Sato, S., Sumida, K., Hiramura, D., Matsushima, A., Tominaga, Y., Shimohigashi, Y., and Shimohigashi, M.
Peptide Science 2004      2005
Structural Characteristics of Drosophila Estrogen-Related Receptor Ligand Binding Domain to Capture the Peptide and Non-peptide Ligands
Liu, X., Matsushima, A., Shirasu, N., Tominaga, Y., Shimohigashi, M., Shimohigashi, Y., and Nose, T.
Peptide Science 2004      2005
Triplicate Alternative Splicings that Produce Multiple Variants of the PERIOD Protein in the Silk Moth Bombyx mori by Deletion/Insertion of Peptides
Takeda, Y., Shirasu, N., Sato, S., Matsushima, A., Chuman, Y., Tominaga, Y., Shimohigashi, M., and Shimohigashi, M.
Peptide Science 2004      2005
Conformation Change-senseing Assay Using Monoclonal Antibody for N-terminal Octapeptide Repreat Region of Prion Protein
Kawano, M., Honda, T., Horiuchi, Y., Yokotani, S., Matsushima, A., Nose, T., and Shimohigashi, Y.
Peptide Science 2004      2005
Immunocytochemical Identification of Mature Peptide Formation for the Circadian Neuromodulator PDF in the Housefly Musca domestica
Sumida, K., Honda, T., Matsushima, A., Tominaga, Y., Shimohigashi, Y., and Shimohigashi, M.
Peptide Science 2004      2005
Immunocytochemical Studies on the Circadian Neuropeptide PDF in the Cricket Gryllus bimaculatus
Shimohigasi M., Honda T., Matsushima A., Sumida K., Meinertzhagen, I.A., Shimohigasi Y., and Tominaga Y.
Peptide Science 2004      2005
cDNA Cloning and in situ hybridization studies of sulfated peptide, sulfakinin, on housefly Musca domestica
A. Matsushima, S. Yokotani, Y. Shimohigashi, Y. Tominaga, M. Shimohibashi
Peptide Science 2003      2004
Localization Profiles of circadian Neuropeptide PDF in Brain of the Cricket Gryllus bimaculatus
T. Honda, Y. Chuman, A. Matsushima, Y. Nose, K. Sakaguti, H. Onoue, Y. Tominaga, Y. Shimohigashi, and M. Shimohigashi
Peptide Science 2003      2004
Two Distinctly Different Isoforms with/without Pentapeptide Fragment in the Silk Moth Clock protein peptide
Y. Takeda, N. Shirasu, S. Sato, A. Matsushima, Y. Chuman, M. Shimohigashi, and Y. Shimohigashi
Peptide Science 2003      2004
Effect of the Phe-Phenyl H→F subsutitution in the Sea Hare Aplysia Neuropeptide H-Asn-d-Phe-NH2 on the Conformation and Contraction Activity
S. Yokotani, A. Matsushima, T. Nose, Y. Shimohigashi, F. Morishita
Peptide Science 2003      2004
A. Kaneki, A. Matsushima, Y. Takeda, S. Yokotani, M. Ozaki, M. Shimohigashi, and Y. Shimohigashi
31. M. Kawano, Y. Horiuchi, T Honda, S. Yokotani, A. Matushima, T, Nose, and Y. Shimohigashi
Peptide Science 2003      2004
Structural Requirements of the N-terminal Octapeptide Repeat of Prion Protein
31. M. Kawano, Y. Horiuchi, T Honda, S. Yokotani, A. Matushima, T, Nose, and Y. Shimohigashi
Peptide Science 2003      2004
Structural characterization of the house fly PDF, a circadian rhythm pacemaker hormone
A. Matsushima, T. Nose, Y. Tomioka, M. Shimohigashi, Y. Shimohigashi
Peptide Science 2002      2003
Localization Profiles of the Precursor Protein of Circadian Rhythm Pacemaker Hormone PDF in Gryllus Brain
T. Honda, Y. Chuman, A. Matsushima, D. Asai, T. Nose, K. Sakaguchi, H. Onoue, Y. Tominaga, Y. Shimohigashi, and M. Shimohigashi
Peptide Science 2002      2003
The Structure-activity Studies of Drosophila FMRFamide-related Peptides
T. Tokunaga, M. Ohtani, A. Matsushima, T. Nose, M. Shimohigashi, S. Aimoto, and Y. Shimohigashi
Peptide Science 2002      2003
Structural Analysis of PERIOD-PAS Domain Binding Regions in Insect Clock Protein TIMELESS
S. Sato, H. Oka, A. Matsushima, Y. Tominaga, Y. Shimohigashi, and M. Shimohigashi
Peptide Science 2002      2003
Structural Characterization of the House fly PDF, a Circadian Rhythm Pacemaker Hormone
Peptide Science 2002   333-336    2003
Structure and Function of Nuclear Localization Signal Peptide Present in Circadian Rhythm Hormone Peptide Precursor
Matsushima, A., Chuman, Y., Sato, S., Tominaga, Y., Shimohigashi, Y., and Shimohigashi M.
Comp. Biochem. Physiol.      2002
cDNA Cloning of PDF Peptide Precursors in Housefly and Last Summer Cicada
Sato, S., Matsushima, A., Chuman, Y., Tominaga, K., Shimohigashi, Y., and Shimohigashi, M.
Peptide Science 2001      2002
Contractile Activity of Drosophila FMRFamide-Related Peptides in the Meretrix Lusoria Heart Muscle
Tokunaga, T., Ohtani, M., Matsushima, A., Chuman, Y., Nose, T., Shimohigashi, Y., Aimoto, S., and Shimohigashi, Y.
Peptide Science 2001      2002
Design and Preparation of Universal Anti-PERIOD Antibodies and their Immunoresponses
Asai, D., Tokunaga, T., Matsushima, A., Sato, S., Chuman, Y., Nose, T., Tominaga, Y., Shimohigashi, Y., and Shiimohigashi, M.
Peptide Science 2001      2002
cDNA Cloning and Nuclear Localization of the Circadian Neuropeptide Designated as Pigment-Dispersing Factor PDF in the Cricket <I>Gryllus bimaculatus</I> (共著)
J.Biochem.   131, 895-903    2002
Design and Preparation of Universal Anti-PERIOD Antibodies and their Immunoresponses (共著)
Peptide Science 2001   399-402    2002
Contractile Activity of <I>Drosophila</I> FMRFamide-Related Peptides in the <I>Meretrix Lusoria</I> Heart Muscle (共著)
Peptide Science 2001   167-170    2002
cDNA Cloning of PDF Peptide Precursors in Housefly and Last Summer Cicada (共著)
Peptide Science 2001   139-142    2002
Structure and Function of Nuclear Localization Signal Peptide Present in Circadian Rhythm Hormone Peptide Precursor (共著)
Peptide Science 2001   159-162    2002
cDNA cloning of a Circadian Rhythm Pacemaker Hormone PDF of theHouse Fly Musca domestica
Matsushima, A., Cuman, Y., Sato, S., Shimohigashi, Y., Tominaga, Y., and Shimohigashi, M.
Comp. Biochem. Physiol.      2001
PERIOD Clock Protein of the Summer Cicada Meimuna opalifera: cDNA Cloning and Structural Analysis
Sato, S., Chuman, Y., Matsushima, A., Shimohigashi, Y., Tominaga, Y., and Shimohigashi, M.
Comp. Biochem. Physiol.      2001
Circadian Rhythm Pacemaker Neuropeptide 'PDF' in Nocturnal Insect Cricket Gryllus bimaculatus: cDNA Cloning, mRNA Expression, and Nuclear Localization
Chuman, Y., Matsushima, A., Shimohigashi, Y., and Shimohigashi M.
American Peptide Society 2001      2001
Gene Expression of a Circadian Pacemaker Hormone Peptide PDF in Cricket Gryllus bimaculatus,
Matsushima, A., Chuman, Y., Onoue, H., Ito, Y., Tomioka, K., Tominaga, Y., Shimohigashi, Y., and Shimohigashi M.
Peptide Science 2000      2001
cDNA Cloning of Circadian Rhythm Pacemaker Neuropeptide PDF in Gryllus bimaculatus and Its Neuclear Localization
Chuman, A., Matsushima, T., Nose, Y., Shimohigashi, and M., Shimohigashi
Peptide Science 2000      2001
Activity Enhancement by Introduction of Two Different Halogen Atoms into Phe-2-phenyl Group of Thrombin Receptor Tetherd-Ligand Analogs
Fujita, T., Inoue, N., Matsuhima, A., Nose, T., Costa, T., and Shimohigashi, Y.
Peptide Science 2000      2001
Molecular Cloning and Gene Expression of a Circadian Neuropeptide, PDF, in the Cricket <i>Gryllus bimaculatus</i> (共著)
Comp.Biochem.Physiol   127, 374    2001
Activity Enhancement by Introduction of Two Differnet Halogen Atoms into Phe-2-phenyl Group of Thrombin Receptor Tetherd-Ligand Analogs (共著)
Peptide Science 2000   135-138    2001
cDNA Cloning of Circadian Rhythm Pacemaker Neuropeptide PDF in <I>Gryllus bimaculatus</I> and Its Neuclear Localization (共著)
Peptide Science 2000   59-62    2001

Association Memberships

 
Japan Society of Endocrine Disrupter Research , THE SOCIETY FOR DISCRETE VARIATIONAL Xα , The Japanese Peptide Society

Research Grants & Projects

 
Structure-Function Studies on Circadian Rhythm Pacemaker Hormones
Grant-in-Aid for Scientific Research
Project Year: 2001